KEY TAKEAWAYS
- The study aimed to evaluate how an IDH1 mutation impacts glioma cell invasion and angiogenesis.
- Results showed that IDH1 mutation drives glioma cell migration, invasion, and angiogenesis through BMP2 signaling.
Hui Xu and the team aimed to assess the impact of an isocitrate dehydrogenase 1 (IDH1) mutation on invasion and angiogenesis in human glioma cells, with significance for brain cancer.
Doxycycline was used to induce mutIDH1 expression in glioma cells. Transwell and wound healing assays assessed cell migration and invasion. Western blotting and immunofluorescence measured protein expression levels.
The study evaluated BMP2’s impact on invasion, angiogenesis-related factors, BMP2-related receptor expression, and Smad signaling pathway proteins following BMP2 treatment. Differential gene expression and transcriptional analysis were also performed.
Results showed successful infection with recombinant lentivirus expressing mutIDH1. The IDH1 mutation enhanced glioma cell migration and invasion, while positively regulating vascularization-related factors and BMP2-related receptors. BMP2 further promoted migration, invasion, and angiogenesis in mutIDH1-glioma cells, likely through BMP2-induced changes in Smad signaling pathway factors.
Post-BMP2 treatment, differential gene expression in mutIDH1-glioma cells was linked to cell migration, adhesion, and Smad-related proteins. KEGG analysis revealed associations with the BMP and TGF-β signaling pathways and cell adhesion. Gene ontology and genome encyclopedia enrichment analyses supported these pathway correlations.
The study concluded that the IDH1 mutation promotes glioma cell migration, invasion, and angiogenesis through the BMP2-driven Smad signaling pathway. Additionally, BMP2 alters transcriptional patterns in mutIDH1 glioma cells, enriching gene loci related to these processes.
This work was supported by the National Natural Science Foundation of China, the Key Research and Development Program of Ningxia and Natural Science Foundation of Ningxia Province.
Source: https://link.springer.com/article/10.1007/s11060-024-04789-x
Xu, H., Cao, Y., Ruan, J. et al. (2024). “The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.” J Neurooncol (2024). https://doi.org/10.1007/s11060-024-04789-x
