KEY TAKEAWAYS
- The study aimed to analyze the regulatory correlation of circUBE2D2 (hsa_circ_0005728) and OC progression.
- CircUBE2D2 localizations and expression patterns demonstrated that silencing inhibits OC progression in malignant phenotypes of SKOV-3.
RuiXue Yanand and the team investigated circUBE2D2 (hsa_circ_0005728) and its role in promoting ovarian cancer (OC) progression, which indicated that its abnormal upregulation is linked to cancer advancement.
Researchers examined CircUBE2D2, miR-885-5p, and HMGB1 by RT-qPCR or WB. SKOV-3 cell functions (viability, apoptosis, migration, and invasion) were evaluated and validation of the results was performed using the CCK-8, flow cytometry, scratch assay, and transwell assay, respectively.
Experimental evaluation showed the correlation between the expression levels of circUBE2D2, miR-885-5p, and HMGB1 assessed by RT-qPCR and Western blotting. Mechanistic interaction was observed between CircUBE2D2 and miR-885-5p whereas miR-885-5p can directly target HMGB1.
Inhibiting the induction of circUBE2D2 or miR-885-5p suppressed OC cell activities, yet these effects were counteracted by down-regulating miR-885-5p or HMGB1 induction. Additionally, knockout of circUBE2D2 reduced tumor growth.
The study concluded that HMGB1 expression is regulated by CircUBE2D2 as it acts like a ceRNA sponge for miR-885-5p. Hence promoting the OC cell proliferation and migration and inhibiting the apoptosis. CircUBE2D2 targeted treatment strategies could offer novel potential courses of treatment for OC.
No Funding was provided.
Source: https://pubmed.ncbi.nlm.nih.gov/38848634/
Yan R, Zeng S, Gao F, et al. (2024). “CircUBE2D2 regulates HMGB1 through miR-885-5p to promote ovarian cancer malignancy.” Clinics (Sao Paulo). 2024 Jun 6;79:100391. doi: 10.1016/j.clinsp.2024.100391. PMID: 38848634.
