KEY TAKEAWAYS
- The study aimed to examine METTL3’s role in cervical cancer and its regulation of Myc.
- The results showed that METTL3 promotes cervical cancer development by modifying Myc through m6A.
Cervical cancer is a prevalent gynecological malignancy with high global mortality rates. METTL3, a vital component of methyltransferase, plays significant roles in various biological functions. However, limited research exists on METTL3’s role in cervical cancer.
Yanyan Ma and their team aimed to investigate the role of METTL3 in cervical cancer and its influence on Myc through N6-methyladenosine(m6A) modification.
Researchers retrieved cervical cancer gene expression data from the Gene Expression Omnibus (GEO) database and analyzed METTL3 and Myc expression levels. They performed METTL3 knockdown in HeLa and SiHa cells and used CCK-8 assays to evaluate cell viability. They also performed real-time quantitative PCR (qPCR) to detect the expression of METTL3 and Myc, and used Western blot to determine protein levels.
Transwell assays assessed cell invasion and migration, and cell proliferation was measured using EdU assays. RNA methylation immunoprecipitation-qPCR was used to evaluate Myc expression after m6A modification.
The results demonstrated elevated METTL3 and Myc expression in cervical cancer tissues based on the GEO database. Patient with high METTL3 expression had shorter disease-free survival (DFS), while high Myc expression correlated with shorter overall survival (OS). METTL3 knockdown significantly reduced HeLa and SiHa cell viability, proliferation, invasion, and migration.
Moreover, METTL3 and Myc mRNA and protein levels were greatly reduced. The m6A modification of Myc was significantly diminished after METTL3 knockdown.
The study concluded that METTL3 promotes cervical cancer progression by regulating Myc expression through m6A modification.
No funding information was given.
Source: https://pubmed.ncbi.nlm.nih.gov/39327253/
Ma Y, Shi H, Zheng W. (2024). “METTL3 Regulates the Translation of Oncogene Myc through m6A Modification and Promotes the Occurrence and Development of Cervical Cancer.” Discov Med. 2024;36(188):1902-1910. doi:10.24976/Discov.Med.202436188.176
